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GrainGenes Sequence Report: BG604834

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Sequence
BG604834
Contig
NSFT03P2_Contig18537
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC307234
wEST map position
BG604834
NCBI UniGene
Ta.54300
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001057724.1 Os06g0507200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AL
7AS
7DS
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0943_G09_N17ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0943_G09_N17, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0943_G09_N17
Probe
WHE0943_G09_N17
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.42864.2.S1_at240WHE2AFFY
[ Show all 21 ]
DNA
tgcgcagctgtacactacctgtagccagggctacgggcaatgccagcagc
agccgcagccacagccgcagatgaacacatgcgctgctttcttgcagcag
tgcatccagacaccatatgtccagtcacagatgtggcaggcaagcagttg
ccagttgatgcggcaacagtgctgccagccgctggccc

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