GrainGenes Sequence Report: BG604541

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Sequence

BG604541

Contig

Ta.1967.2.A1_x_at

TaAffx.104812.1.S1_s_at

NSFT03P2_Contig13851

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001067015.1 Os12g0560200 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat vernalized crown cDNA library

Tissue

Crown tissue of seedling

Developmental Stage

Five-week old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0834_H09_O18ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0834_H09_O18, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE0834_H09_O18

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ccctggcgaccatcatgtgggtcacgtcggcgcatcacgccgccgtcaac
ttcgggcagtaccccatggccgggtacatccccaaccgcccgaccatggc
ccggatgaacatgccgaccg


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