GrainGenes Sequence Report: Ta.8060.1.S1_at
Sequence
BG604484
Contig
Ta.8060.1.S1_at NSFT03P2_Contig9303
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC298837
NCBI UniGene
Ta.8060
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wlsu2.pk0001.h3:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0834_C01_E02ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0834_C01_E02, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0834_C01_E02
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gaggaggaggaagcctgggtgaacagggggagctcgccgcggagaagcac
gtccgctacatcgtcaccgcggagaagaagaaggactcgttcgagtcgct
ggtgatggagcacctccgggccagcggcgcctactggggcctcaccacgc
tcgacctcctccacaagcttcacgccgtggacgccgccgaggtcgtcgac
tggatcatgtcgtgctaccacccggaatctggtgggttcggggggaacgt
ggggcacgacccgcatgtcctctacacnctcagcgccgtgcaggtcctct
gcctcttcgatcggctcgatgttcttgatgcagacaaaattgctgactat
attactggacttcaaaatgaggatggatcattttctggtgatatttgggg
tgaagttgatact
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.8060.1.S1_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||