GrainGenes Sequence Report: BG415616

HVSMEk0007D02f Hordeum vulgare testa/pericarp EST library HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA clone HVSMEk0007D02f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HVSMEk0007D02f

Probe

[Bcl271ct619cn2536]

{SpCl-312}

BG415616

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 133; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 471.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were raised from seeds in a Controlled Environments growth chamber maintained in continuous light at 18oC, and testa and pericarp were dissected from developing kernels at Washington State University, Pullman, WA (Kannangara, von Wetstein). Total RNA was prepared, poly(A) RNA was purified, one cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were raised from seeds in a Controlled Environments growth chamber maintained in continuous light at 18oC, and testa and pericarp were dissected from developing kernels at Washington State University, Pullman, WA (Kannangara, von Wetstein). Total RNA was prepared, poly(A) RNA was purified, one cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

tcttgcggcagaagcaaagcgcggcgaggcgaggcgagtcggcggcggca
agatggacggcggaggcgaggaagggaagcagcacccgcagctggtgctg
gcgcacaagctgttcctcctctcgcagccagacgtcgacgacctcgccaa
ggtcggcctccgcgacgacgtcctcgccgcagtgaaatccgatgacatgg
cggcgctgtacgagtctctggcggccgacggcgtgctggatatggacgcg
gctctgctcgcggatatgcgcggcgggatcgaggaggacacccgcaagtt
ctacgagaatattgcggatgctgaagagattttgggtgagagtgaagtgc
gtgaaccccatctagccaaatccttatatttcataggggttggcgagaag
gaaaaagcgctggaccaccttaaagtctctgaaggccatactgtagctgt
cgggcaaaagatggaccttgttttctacactttacagatggccctttccc
atatggattttgatctcatcacaaagtctgttgacaaggctaaaattttg
ttagaaaagggtggtgattggaagaagaaaaacatggtcaaagttgttga
aggcttgctcctg


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