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GrainGenes Sequence Report: BG367911

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Sequence	BG367911
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC170516
NCBI UniGene	Hv.2132
DB Remark	Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, moderately similar to NP_001046696.1 Os02g0324300 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Hordeum vulgare subsp. vulgare
Cultivar	Morex
Clone Library	Hordeum vulgare 20 DAP spike EST library HVcDNA0010 (20 DAP)
Tissue	20 DAP spike
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	HVSMEi0014J12f Hordeum vulgare 20 DAP spike EST library HVcDNA0010 (20 DAP) Hordeum vulgare subsp. vulgare cDNA clone HVSMEi0014J12f, mRNA sequence.
Strain	lab_host SOLR vulgare
Clone	HVSMEi0014J12f
Probe	kr68B0303
Remark	DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 438; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 513. Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 20 DAP (Fenton ). Total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Choi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http: Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 20 DAP (Fenton). Total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Choi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:
DNA	ggagcctccgcacctacgctcgtcgcgccgctgatatcctcgtcctcctc gccggagacaacatgtcgacgcttgccgcggcgagggccgacaacttcta ctacccgcccgaatggagcccgaagaagggtgggctgaacaagttccacg gccagcatgccctcagggagcgggcgaggaagctggaccagggcatcctg attataagattcgagatgcctttcaatgtctggtgtggtggatgcagttc catgatagggaagggtgtaaggtttaatgctgaaaagaaacaagttggaa attactactctaccaagatatggagcttcagtatgaaatcaccatgttgc cagcatgagatagtcatacatacggacccgaagaacactgagtatgttat agtcagtggagctcagcggaagactgaagattttgatgttgaagatgcag agacgctcctgctccctgcggatgaagaaagagancagcttgcggatcct atgtataagctcgaacaccagggagaggatattcggaagaagaaagaaga ggagcctgttttagttcggcctcaacgcctctctgatagccggcattccg gagattattccctgaacaggaccttccgcgaccgccttaagagccaaaag aagagagttgctgaagagaagaaatcagcgaggaagaggggactggaagt acgccttcttggcccccttcccaaaagatgccggcgcaccaccccgggtg aggttcgctctgaatttcggaaaagcgaaaggccagaaagcggggatcag gctctcttcttttccagaaacttccgtttaacttaaaggcagaatacccg cgccgaaaggcgaataaaaactagg

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