GrainGenes Sequence Report: BG365474

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Sequence

BG365474

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus, strongly similar to NP_564011.1 UBC13B/UBC36 (UBIQUITIN-CONJUGATING ENZYME 36); ubiquitin-protein ligase [Arabidopsis thaliana]'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

Morex

Clone Library

Hordeum vulgare 20 DAP spike EST library HVcDNA0010 (20 DAP)

Tissue

20 DAP spike

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HVSMEi0002N11f Hordeum vulgare 20 DAP spike EST library HVcDNA0010 (20 DAP) Hordeum vulgare subsp. vulgare cDNA clone HVSMEi0002N11f, mRNA sequence.

Strain

lab_host SOLR

vulgare

Clone

HVSMEi0002N11f

Probe

[Bcl685ct1187cn4469]

{SpCl-89}

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 430; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence start: 15; High quality sequence stop: 736.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 20 DAP (Fenton ). Total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Choi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 20 DAP (Fenton). Total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Choi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

gttcgctgacgccgcttcctttggtacttccccttcctcctcccctcccc
tcccctccgccgcgatcgaccacgaaaccctagccctagccccgccatgg
ccaatagcaacctcccccgccgcatcatcaaggagacgcagcggctgctc
agcgagccagcgtggggggatcagcgcttcaccatcggaggagaacatgc
gctacttcaatgtcatgatccttggcccaacgcagtcgccgtatgaagga
ggagtattcaaacttgagttgtttttacctgaagaatatccaatggctcc
gccaaaggttcgctttctcacaaaaatttaccatccaaacattgacaagc
ttgggaggatatgccttgatatcttaaaggacaagtggagtcctgccctt
cagatccgaacagttcttttgagtattcaagcactcctcagtgctccaaa
tcctgatgatccgcttgctgacaatgtggcaaagcactggaaagccaacg
agacagaagctgtggaaacagccaaagggtggacccgtgtctatgctaaa
gatggctgaactctggcttgctatgaccgagggctcattgatgttggtga
ggtggaaggcgaaatcagcagatcagggtggtctgaaagagagaagtcgg
gagagaactattgaaaaaggaaaggttcgacaagtgaagtgggggccaag
aggatgtatatctagaccccggagtgggatgggttggagcccgcgtccag
tgattggccgggaggagccagcccagattaacatgcgcggagaagaagag
ggcatgtgccgaggctggatagagagctac


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