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GrainGenes Sequence Report: BG342887

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Sequence	BG342887
External Databases	Data at GenBank Data at EMBL Data at DDBJ
DB Remark	Locus Source: Hordeum vulgare subsp. vulgare
Keyword	EST
Species	Hordeum vulgare subsp. vulgare
Cultivar	Morex
Clone Library	Hordeum vulgare pre-anthesis spike EST library HVcDNA0008 (white to yellow anther)
Tissue	pre-anthesis spike
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	HVSMEg0001C01f Hordeum vulgare pre-anthesis spike EST library HVcDNA0008 (white to yellow anther) Hordeum vulgare subsp. vulgare cDNA clone HVSMEg0001C01f, mRNA sequence.
Strain	lab_host SOLR vulgare
Clone	HVSMEg0001C01f
Probe	BG342887
Remark	DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: On Feb 27, 2001 this sequence version replaced gi:13155216.; Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 258; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 701. Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spike with awns trimmed were collected at white, green and yellow anther stages (Fenton). Total RNA was prepared from each pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close lab (Choi) at the University of California, Riverside. Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing) Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch , Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http: Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spike with awns trimmed were collected at white, green and yellow anther stages (Fenton). Total RNA was prepared from each pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close lab (Choi) at the University of California, Riverside. Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing) Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:
DNA	gagcagctagctagcacagtcccctaccaccaccaccaccagcccactct atcagcctcgggtgaagccgagcagatccatggcgcagtctcgtcgcatg gccgggtccgccctcctgctgctgctcctcctcgtcgccacagagatggg ggcgacgacagtgaacgtggcggaggcgcgggactgcctgtcgcagagcc acaagttcaagggcacgtgcctcagcagcagcaactgcgccgccgtctgc cgcaccgagaacttccccgacggccagtgccacacccagaacctcgcccg caagtgcttctgcaagagggtctgctagatctgttgctgctgttggtcag tgccgttcgctagatctgttcgtcagtagctgcctgcagttcgtgtgact gttgccccgtaataaagttaggacatcaataaccgggggctccgtagttt gtgttcccccgcacgtcgtgtttgccgatgcttcgtgtgggtataaagga acaagggcgttggtcacacagggctttccctggggggtattgggaggcat cctcaaaacgaactccttttatttttgaaaaaaagaatttcttcaaaaaa aaaaaaaaaacactcggggggggggctctggttccccattgtggccttat gaccaggggccaaacgaaaaaagctgggccgattttttttaacaaatttt ttggggaatatccctcacttgaaagggctttactgttggttttggatccc accatttgtggaaggcccgggaaatttccaggtggggggtttgttatttt gataccaacccttttgaaccacacttttggcggatgttgttttn

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