GrainGenes Sequence Report: BG314142
Sequence
BG314142
Contig
Ta.7436.2.S1_at NSFT03P2_Contig16171
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Germplasm
T. monococcum ssp. monococcum DV92
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum early reproductive apex cDNA library
Tissue
Early reproductive apex
Developmental Stage
Seven week-old plants
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2459_F07_K13ZS Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2459_F07_K13, mRNA sequence.
Other Name
WHE2459_F07_K13ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli XLOLR
DNA Library
TM043E1X
Clone
WHE2459_F07_K13
Probe
CFE36 CFE37
Remark
DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tgggggtgtgcgccaacgtgctcaacgggctgatcaacctgcagctgggg
acgccgccgaagcagccgtgctgctcgctaatccaggggctcgccgacct
ggaggcggcggtgtgcctctgcacggcgctcaaggccaacatcctgggca
tcaacctcaacgtgcccatcgacctcagcctcctcgtcaactactgcggc
aagaacgtcccctccggcttccagtgcccccgctagaagagaagacccga
gaagatccatcgtccgctgcatgcaggcatccactatacgtac

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GrainGenes Sequence Report: BG314142
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