Query (optional)   in Class  

GrainGenes Sequence Report: TaAffx.3444.1.S1_at

[Submit comment/correction]

Sequence
BG314090
Contig
TaAffx.3444.1.S1_at
NSFT03P2_Contig5640
Tracefile
[ Download ]
[ View ]
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BG314090
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Germplasm
T. monococcum ssp. monococcum DV92
Species
Triticum monococcum
Cultivar
DV92
Chromosome
1AL
1BL
1DL
Clone Library
Triticum monococcum early reproductive apex cDNA library
Tissue
Early reproductive apex
Developmental Stage
Seven week-old plants
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2459_A09_A17ZS Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2459_A09_A17, mRNA sequence.
Other Name
WHE2459_A09_A17ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli XLOLR
DNA Library
TM043E1X
Clone
WHE2459_A09_A17
Probe
WHE2459_A09_A17
Remark
DB_xref: taxon:4568
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.3444.1.S1_at704WHE2AFFY
[ Show all 7 ]
DNA
tcacgaaatttacagcaaaactaaaaagaaacagactgtcccaaagaatc
cccaacactcgtaacacaaacagctcacagtatgcataaaacattctcta
gtgaccaccatcagcgagaactccctcttctgcagctttacctccacaag
accttgcgacagactcaaacagcctctcaatctccggtgcacagcgtgta
aaggatcgagcccaaaagctatagcgtggattctttatgagctcaatgaa
agtaataagtaatccccagggatgaggacgattaactattaggcgctcta
aaagaactctagttatctgctcctgtacgatatcctgtgttgcctcagca
aacaagtgcaaaatgatgaaggagaagtagtgagtatgattgtttggata
gcgtaactggttagcaattgcgtacaggaggaggtaac

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.