GrainGenes Sequence Report: NSFT03P2_Contig12858
Sequence
BG314058
Contig
Ta.25493.1.A1_at NSFT03P2_Contig12858
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Germplasm
T. monococcum ssp. monococcum DV92
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum early reproductive apex cDNA library
Tissue
Early reproductive apex
Developmental Stage
Seven week-old plants
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2467_E11_I21ZS Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2467_E11_I21, mRNA sequence.
Other Name
WHE2467_E11_I21ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli XLOLR
DNA Library
TM043E1X
Clone
WHE2467_E11_I21
Remark
DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ccctctttctttcaggccctaattaacctcgcaaccaaacacatgctcta
tgtcagtgaggtacatcactatcaatactggcgaccgatttgtccaccac
acgaacgttatacagctcgtatacccttgcccggttctcttgccaccatt
gctctgcttgcgtctcgctgaaacgcttccggctgaaccgtaccctccta
agatctctagcccctccaggtagggcagtaagggtgatgtagacccctgg
ttcatcttgctctacccattcagactcaggcttanagtctgaatcaggct
gtttggttccattcttgccaacttccatgtgagtcctatttctaactgaa
cttggtccattataaacaatcaacc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig12858
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||