GrainGenes Sequence Report: NSFT03P2_Contig13273
Sequence
BG314004
Contig
TaAffx.128744.1.S1_at NSFT03P2_Contig13273
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC297845
NCBI UniGene
Ta.54042
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001049173.1 Os03g0182400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat salt-stressed sheath cDNA library
Tissue
Sheath
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2065_G03_N05ZS Wheat salt-stressed sheath cDNA library Triticum aestivum cDNA clone WHE2065_G03_N05, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2065_G03_N05
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin , Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttggttgacacttatagccagacgctcgcgtcattttgctggaaccag
attcatgaaacgaggtgttaatgaaaagggtagggttgccaatgatgttg
agactgaacagattgtttttgaagacacacctgatgacatcccaagtcaa
ataacttctgttgtacaacatagaggatcaatacctcttgtctggtttca
ggaaacttcaagactgaatattaggccagaaatcacgttaaaatcagatg
tggactacaaggccacccgccttcattttgaaaaccttgttcttagatat
gggaaccctatagtcatcttaaacttaatcaagactcgtgagaagaagcc
tcgtgaatctttgctccgtgcagaatttgcaaaggctatccattacataa
ataaagggttaccggatgacaaacgtcttaagtttttacacatggatctg
agtaagctttctcgaaagaaaggcgccaatgtgcttggact

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig13273
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