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GrainGenes Sequence Report: BG313928

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Sequence	BG313928
Contig	Ta.4453.2.S1_at Ta.4453.2.S1_x_at NSFT03P2_Contig11596
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC291469
NCBI UniGene	Ta.53874
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_199924.1 unknown protein [Arabidopsis thaliana]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat salt-stressed sheath cDNA library
Tissue	Sheath
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2066_H04_P08ZS Wheat salt-stressed sheath cDNA library Triticum aestivum cDNA clone WHE2066_H04_P08, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2066_H04_P08
Probe	[Wcl4ct21cn26] BG313928
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin , Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	accatggcgctcatgctcagcccggccgcgaccttcctccccgtcgtcgc cggcaagcccaccaccgccatgcggaacctcctcttcggcaccaccacca gcaccaccagcggtcaccggagcactcgcaaggtggtggcgatggcggac atcctgggcgacttcggcgcgcgggacccgttcccggaggagatcgcgag caacttcggggagaagacgctgggcaacgtggacacgctgcaccgcatcc tcatccccacgctctccgtgctctccctctcccgcgtcccgctcgacgcc gacccggccccgctctccgaggaggacgcccgcaggctgctcttcaaggt ggtcggctggcggctcctcttccccagcaagggcgactccgacgtgctca agctcgagtgcgtctggaaggtccgcgaccaggcctgcggcgacgagctc gtcgccaggatcaacaaggcgctcgacggcgccggccacgcccccgccgc gctccggttcgaggcgcccaaccaagtc

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