GrainGenes Sequence Report: Ta.1973.1.S1

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin , Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA Homology

Ta.1973.1.S1_at	882	WHE2AFFY
[ Show all 7 ]

DNA

cagttgagctaataggcgatggattcccaggcgccggccggcgacgaccg
ccggagcacccacctcaacaaggctgtggacgagcaatctcctcgacaat
cggcgcccagcaagcaggcaggtgctccgtgcgtctacctcaagaaaggc
acgtgcgcggagggaagcatgcggcatgacgcccatggccacgcgcccca
ccttgccgcaagccatgctcactgagcgtaccgtatagcagcagccagca
gcagagacaatactatgtattgccgcaataccagtactacgtactttcta
cgtatttctgctccgtcatgtcttcttctcttccgctgcttgctgctagt
atctatctaaggccgccccatatggccgtatatacagtaaaaagattgta
ataaataatgacccactctgtgtgtggtccctgtgattgcggtca


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.