GrainGenes Sequence Report: BG299898

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Sequence

BG299898

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

Morex

Clone Library

Hordeum vulgare seedling shoot EST library HVcDNA0001 (Cold stress)

Tissue

Seedling shoot

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HVSMEa0022G07f Hordeum vulgare seedling shoot EST library HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare cDNA clone HVSMEa0022G07f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HVSMEa0022G07f

Probe

[BG299898.1]

{SpCl-236}

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 102; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence start: 2; High quality sequence stop: 384.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated at 5oC for 2 days. Shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 600000 pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations , DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main ). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated at 5oC for 2 days. Shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 600000 pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

ccccgggctgcaggattcggcgcgagagagagagagagagagagagagag
agagagagagagagagagagagagagagagagagagagagagagagagag
gccgcctggggctggctgagctgtcccaccccccacctcccaccaacctc
tctgcgtgctccctcccccgattcctgcttccccaattcagccgcagcga
ggagtaggatcgcgcctcctggtctagcccggacccggcagactccttac
ttgccccggcagagcaggcggaatcggtgtgccgtcgtcgggcgcagaga
gattcttggggctgctgagacctgcgatgtcttgctagctcgacgtgggt
ttgtgtcgtgggagtaggcgagagcggtagtacagcactgccggctaagt
ctgctcggctgattctttgcttgagcgggaggcatataagtgcgtgccat
gagggagatgatgtgcgccagcgggtaagctatcttcgcggccgccgtga
gggagaacgtctgtgctgcgagaactgtatcagggaggcgctaaaggttg
accaacgggaggcgccgtactatggtatcctggtgagaaaagggtgaaaa
acagagacgcgcttgcgcggcgctctcgtgggccccggtcgagctggatc
gggagatgtatcgagggttgtcatgcgagccttgttcaccccgcgtgaat
ccctcaggataactcgaaatattgatctctgtccgccccgcgcttagcgc
atggaacacgcaggtgtcttgttgaatgcacgatgacgggccgtagggct
gggcctgggacagcgggtaccgcgccccgctggctccccgatagtgttgg
tgt


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