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GrainGenes Sequence Report: Ta.21072.1.A1_at

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Sequence
BG275030
Contig
Ta.21072.1.A1_at
NSFT03P2_Contig11068
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC287620
wEST map position
BG275030
NCBI UniGene
Ta.21072
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001060734.1 Os07g0693900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AS
2BS
2DS
Clone Library
Wheat salt-stressed crown cDNA library
Tissue
Crown
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2120_H08_P16ZS Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2120_H08_P16, mRNA sequence.
Other Name
WHE2120_H08_P16ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA038E1X
Clone
WHE2120_H08_P16
Probe
WHE2120_H08_P16
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.21072.1.A1_at694WHE2AFFY
Ta.29360.1.A1_a_at442WHE2AFFY
TaAffx.118885.1.S1_at76WHE2AFFY
DNA
tggatgcatgtgctgcctgcttcgagcaaaggacaacattcactcagcaa
gttttggcaaaagcactaaaccaactggttgaacagattcccctaccttt
gctgttcatgcgaactgttatgcaagcgataagtgcatttcctgccttgg
tggattttgtgatggagatcatgttacgccttgttagcaagcagatatgg
aaatatccgaagttatgggtaggattcctgaagtgtgctatcctgacaaa
gcctcagtcctatggtgtgttactgcagttaccagctccacaactcgaaa
acgccctgaataagaaccctgtactgaaagcgcccttggtcgagcatgcc
agccagccaaacgtgcgatcgactctcccaaagtct

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