GrainGenes Sequence Report: BG274662

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Sequence

BG274662

Contig

NSFT03P2_Contig16329

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

BG274662

DB Remark

Locus Source: Aegilops speltoides

Keyword

EST

Species

Aegilops speltoides

Cultivar

F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1 )

F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)

Chromosome

7AS

7BS

Clone Library

Aegilops speltoides anther cDNA library

Tissue

Anther

Developmental Stage

Premeiotic anthers

Data Source

genbank	Release 135, Apr 15 2003
genbank	Downloaded 2008-2009

Title

WHE2237_E07_I13ZS Aegilops speltoides anther cDNA library Aegilops speltoides cDNA clone WHE2237_E07_I13, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE2237_E07_I13

Probe

WHE2237_E07_I13

Remark

DB_xref: taxon:4573

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons , Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA Homology

Ta.3199.1.S1_a_at	325	WHE2AFFY
Ta.3199.1.S1_a_at	234	WHE2AFFY
Ta.9143.1.S1_at	64	WHE2AFFY
Ta.9143.1.S1_at	52	WHE2AFFY
Ta.23044.1.A1_s_at	58	WHE2AFFY

DNA

ttcgcgggcgccacgagcacttccccggcacgctccccaccgggtccgac
gtccagtcgctcgtcctcgagttcatcatcaccttctacctcatgttcgt
catctccggcgtcgccaccgacaacagagccatcggggagctggcagggc
tggccgtgggtgcaaccatcctgcttaatgtgctgatagctgggccggtg
tcgggggcgtcgatgaacccggcgaggacggtggggccggcgctggtggg
gagcgagtacaggtcgatctgggtgtacgtggtggggc


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