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GrainGenes Sequence Report: BG274294

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Sequence	BG274294
Contig	NSFT03P2_Contig17415
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BG274294
DB Remark	Locus Source: Aegilops speltoides
Keyword	EST
Species	Aegilops speltoides
Cultivar	F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1 ) F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)
Chromosome	1AL
Clone Library	Aegilops speltoides anther cDNA library
Tissue	Anther
Developmental Stage	Premeiotic anthers
Data Source	genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title	WHE2226_H03_O06ZS Aegilops speltoides anther cDNA library Aegilops speltoides cDNA clone WHE2226_H03_O06, mRNA sequence.
Other Name	WHE2226_H03_O06ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	AS040E1X
Clone	WHE2226_H03_O06
Probe	WHE2226_H03_O06
Remark	DB_xref: taxon:4573 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons , Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.3225.1.S1_at 807 WHE2AFFY Ta.3225.1.S1_x_at 807 WHE2AFFY Ta.23108.3.S1_x_at 48 WHE2AFFY
DNA	tcaacgaggacttctacaagcgcacgctctccggcggcgtcgtcacgctc atctccgccctcgtcatgctgctcctctttgtctccgagaccaggtcgta ttattattcagcaaccgagactaagttagtggtagacacatcaagaggag aaaggctaagagtaaattttgatattactttcccgagtattccttgcacc cttctcagtgttgatacaagggatattantggcgagcagcggcaggacat taggcatgacattgaaaagaaaagattggactcacatggtaatgtcattg aatcaagaaaagagggcattggtggaacaaagatcgagaaaccattgcaa aaacatggtggaaggcttggcaaaggcgaggaatattgtggcacctgtta tggtgcagaggagtcagatgaacaatgctgtaattcatgtgaagaagtta gggaggcctacaagaagaaagggtgggctcttacaaaccctgacctaatc gac

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