GrainGenes Sequence Report: BG274019
Sequence
BG274019
Contig
NSFT03P2_Contig5171
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BG274019
DB Remark
Locus Source: Aegilops speltoides
Keyword
EST
Species
Aegilops speltoides
Cultivar
F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1 ) F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)
Chromosome
2AL 2BL 2DL
Clone Library
Aegilops speltoides anther cDNA library
Tissue
Anther
Developmental Stage
Premeiotic anthers
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
WHE2232_E05_J10ZS Aegilops speltoides anther cDNA library Aegilops speltoides cDNA clone WHE2232_E05_J10, mRNA sequence.
Other Name
WHE2232_E05_J10ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
AS040E1X
Clone
WHE2232_E05_J10
Probe
WHE2232_E05_J10
Remark
DB_xref: taxon:4573 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons , Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.6277.2.S1_at 787 WHE2AFFY
DNA
caccgctccccgccccctcgaatggatcccgctccggcgacccctcgctg
gaacctggagcgcccctacctcaccggacgcttccaccaggaggcgaagg
tgtccgcgccgtcgcaggcgcccggatccaagtctttctctctcgactcc
ttcagccgcggcgctggcggcggcgctgggagtgtcctcggatcgtacgc
tgtttctgtgcaggagcttttagttattgatgatttgctatcagctctgg
tgggtattgagggaagatacatttcaattaaaagagtacgcggaaaggag
ggatatgttgtcttccagattgattcttcaatggaccttgcattccagga
gttgactcgccgaattttccccttatgtgaagatttcgtgctagcaagcc
agtttgtggagtccaggtcacatttcaaaaatggtttggtcaaccatgct
ctagcggcagctct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BG274019
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||