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GrainGenes Sequence Report: BG263742

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Sequence	BG263742
Contig	Ta.6428.1.S1_s_at Ta.6428.2.S1_a_at NSFT03P2_Contig15701
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC292241
NCBI UniGene	Ta.6428
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001064824.1 Os10g0470900 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2348_E02_J04ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2348_E02_J04, mRNA sequence.
Other Name	WHE2348_E02_J04ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA019E1X
Clone	WHE2348_E02_J04
Probe	[BG263742.1] {SpCl-124}
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	agcggcgcaaggaggcggtgatggagtcggatcagggcaagctcttcatc ggcggcatctcctgggagacgacggaggagaagctgcaggagcacttctc caacttcggcgaggtctcccaggccgccgtcatgcgcgacaagctcactg gccgcccgcggggcttcggcttcgtagtctacgccgaccccgccgccgtc gacgccgccctccaggagccccacaccctcgacggccgcacggtcgatgt gaagcgggcgctctcgcgggaggagcagcaggctaccaaggcggtgaacc ctagcgcaggaaggaacgctggaggtggtggcggcggcggcggcggcggc ggcgatgccggtggtgctaggacaaagaagatttttgtgggcggactgcc ctccagtctgacagatgaggagttccggcagtacttccagaccttcgggg ctgtcaccgatgttgtggtgatgtatgaccagacaacac

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