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GrainGenes Sequence Report: BG263683

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Sequence
BG263683
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC338927
wEST map position
BG263683
NCBI UniGene
Ta.32194
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_193327.2 nodulin MtN3 family protein [Arabidopsis thaliana]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AS
4BL
4DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2344_G07_N14ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2344_G07_N14, mRNA sequence.
Other Name
WHE2344_G07_N14ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE2344_G07_N14
Probe
MAG1441
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gtccaccatgctctatctatctatatatatatatggtcatctatgtctat
gtgagagacgtagctagcttgccatcaatttgtacctcccggctcttaca
ccaatggccgatccaagcttcttcgtcggaatcgttgggaacataatctc
catactcgtcttcacatctccaattgcgactttccggagggtcgtgcgga
acaagagccgggaggagttccggtggctgccctacgtcaccaccctcctc
tgcacctccctctgggccttctatggcctcctcaagcccggcggcctcct
catcatcaccgtcaacgccgccggcgccgctctgcaggccacctacgtcg
ccctctacctggcctatgcccccagggacaccaaggtgaagatggcga

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