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GrainGenes Sequence Report: NSFT03P2_Contig14068

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Sequence
BG262882
Contig
Ta.3407.1.S1_at
NSFT03P2_Contig14068
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC331730
wEST map position
BG262882
NCBI UniGene
Ta.54532
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001056479.1 Os05g0589000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL
1BL
1DL
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0948_D12_H24ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0948_D12_H24, mRNA sequence.
Other Name
WHE0948_D12_H24ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
Clone
WHE0948_D12_H24
Probe
WHE0948_D12_H24
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.3407.1.S1_at1086WHE2AFFY
[ Show all 6 ]
DNA
tggttgcagtcagtacttggagccaaggactttatccatttaatgttctc
tttgttgctcgtcacatctcagctgcacttaaagattgctgcgctacctg
tcttctgctgggctcttgatcatgttgccagattcctattgcgtaacttt
tcccgctcatctttctacaggggatacttggaagagccttgcctttgggt
ggagacaaacaacaccacgctcagcctccttagctcgaatgctgaacttg
ccttggggttccttctgatcatatcactgttctcgtggaggcgcaatttc
atccagacattcatgtactggaacgtgctgaagatgatgtaccgcgcgcc
cgtgacatccagctaccaccagagcgcgtgggccaagatcgggcggaccg
tcaacccgtacatcgaccgctacgcaccgttcctccagacacccatctcc
atggtccagcggtggtggctcaggtagcgagcgagacacggaggaatctg
gcatgcgaggcctgtggcggagaagggaaaaacatatactatctactact
actactagtaactagcatcgaccgtcc

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