GrainGenes Sequence Report: BG262864
Sequence
BG262864
Contig
Ta.5204.1.S1_at
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC308439
wEST map position
BG262864
NCBI UniGene
Ta.5204
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001060650.1 Os07g0681100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AS 2BS 2DS
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0948_C02_F04ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0948_C02_F04, mRNA sequence.
Other Name
WHE0948_C02_F04ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
Clone
WHE0948_C02_F04
Probe
BG262864 WHE0948_C02_F04
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.5204.1.S1_at 696 WHE2AFFY
[ Show all 7 ]
DNA
accaggggtgaagatcatactaggaacagcatatggcatcgcgcacatcc
attcagaaggcggcgcaaagctcacccacggcaacatcaagtcgaccaac
gtcctcgtcggtcaggatcaaaacgcgtacgtctcagactacggcctcag
ctctctgatgaacgcgcccgtcagcgcgtcccgcgtcgtggtcggctacc
gcgccccggagaccatcgagaaccggaagagcacgcagaagtcggacgtg
tactgcttcggcgtcctcctcatggagatgctgacggggaaggcgccgct
ccagtcgcaggggaacgacgacgtggtggacctcccgaggtgggtgcact
cggtggtccgggaggagtggacc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BG262864
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