GrainGenes Sequence Report: BF620794

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Sequence

BF620794

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

Morex

Clone Library

Hordeum vulgare seedling shoot EST library HVcDNA0003 (Etiolated and unstressed)

Tissue

Seedling shoot

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HVSMEc0020N23f Hordeum vulgare seedling shoot EST library HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare subsp. vulgare cDNA clone HVSMEc0020N23f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HVSMEc0020N23f

Probe

[BF620794.2]

{SpCl-29}

BF620794

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: On Dec 18, 2000 this sequence version replaced gi:13109747.; Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 186; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 407.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

agtcccgaccaccaccaccgacaggccaatcaatcatggagttccctcac
cgccacggccacggccacggccgccgcgacgacgacgacgacgaccgccg
cccccccgccccgtacggccgccaagagcccgacccctacggcgcccctc
ccccctcctacgggcggcgccccgactacgtcgcctacggcgccccgccc
cccgcctacggtggcggcctccacgactactacggcggtcgggccccggc
ctactgtgggggctgcgaggaccactacggcggtcgcgccccggcctact
gtggggggcgcgaggaccactaccgcagccgctccccggcctactgtggg
gggctcgaggacgactactggcgctacgcgcccgcaccggccgggtaccg
gtggggtgactaccgggcgccacgcgcccgctccggcgtgcgggtgctgc
ggctctactggttcgtttcgccctgccccggcgtcgtgttgtccttcttc
ctctgtctcgcgacggcggggtccttgcgcgtgccccggcgccctcccgt
ccctgacctcgttcgcaggcgcggggtcccgttgtcgcccccggtattcc
catttcatcgccttaccatatgccggataagtgtcctcattctattgctt
ctgtcagcttggggctttttgcctctgctttcttccccttccaccctcgt
ccctgcccctggccgtttttttgttcctcttgtcgtcactgttcccccat
gtctctttcgctggccgtgcttcccgccccgtctggttcctctctttgtt
ccccgcgttccgtcctcg


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