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GrainGenes Sequence Report: BF617604

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Sequence	BF617604
External Databases	Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene	Hv.6427
DB Remark	Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, moderately similar to NP_001042198.1 Os01g0179300 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Hordeum vulgare subsp. vulgare
Cultivar	Morex
Clone Library	Hordeum vulgare seedling shoot EST library HVcDNA0003 (Etiolated and unstressed)
Tissue	Seedling shoot
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	HVSMEc0018E02f Hordeum vulgare seedling shoot EST library HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare subsp. vulgare cDNA clone HVSMEc0018E02f, mRNA sequence.
Strain	lab_host TJC121 vulgare
Clone	HVSMEc0018E02f
Probe	[Bcl1027ct1575cn5280] {SpCl-141} BF617604
Remark	DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: On Dec 18, 2000 this sequence version replaced gi:11881338.; Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 495; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 539. Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http: Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:
DNA	acgagcccacgccgcagcagcagaaggagctcttctgggcgcccgcgccg ctcaacaccaagtcgtgggccgacgtggaggacgacgacgacgacgacta cttcgccaccaccgcgccgccggcgcccatctgggggaacgaccacgcca acgccaagacggccaaggaggaggaggaggacgaggtcgatgccgtccac gccgccctgccggaggaaattgagagtgaggatgaagagcatgatgatga ggctgaggatggtgctgaggatgaacctgagcatgaagtggaagctgctg atcctgaggtgaagaaagctgcagcagcacctaaagagacagaaaggcag ctgtccaaaaaggagttgaaaaagaaggaactggctgaacttgatgctgt gttggctgagttgggaatttctgaaaacgcaagcgatgctgcacaagatg gaaacaacaatactgagaagaaaggtgcgaatcaagctggtgatggagat agaaaggaagacgcacctgccccttcagagagcaagaatgccaagaagaa gaagaacaagaagggacagagcgcaaaggaggcagcaaaagaaagctgag tgtcngaggaggctgccagtgcagaacccgaggaagacacttcgctgtgg atgtcanggagccccctaagaagatggcctcgatgaaaaggaaaaattcg gccagggagacggcacagctggcaagattgccccaacagaggccgcagcc cagaccccgaggctggggggggcaaaaaaaagggaagaacct

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