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GrainGenes Sequence Report: NSFT03P2_Contig17116

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Sequence
BF485004
Locus
XBF485004
Contig
NSFT03P2_Contig17116
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC278901
wEST map position
BF485004
NCBI UniGene
Ta.53887
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001045261.1 Os01g0926300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL
3BL
3DL
5AS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1792_B12_C24ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1792_B12_C24, mRNA sequence.
Other Name
WHE1792_B12_C24ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE1792_B12_C24
Probe
WHE1792_B12_C24
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.9372.1.S1_at539WHE2AFFY
Ta.9372.1.S1_x_at539WHE2AFFY
TaAffx.86703.1.S1_at117WHE2AFFY
TaAffx.86703.1.S1_at82WHE2AFFY
DNA
ccacctactgcagaatgcgttccttctatcaaggaagtcattgctaatgg
catcagcgtcaatgtcactcttatcttctccgttgcaagatacgaggctg
tgattgatgcttaccttgacgggcttgatgcttctggtttgagtgactta
tcccgagtgaccagtgtagcatccttctttgtcagccgagttgacagtct
gatcgacaaaatgcttgagaagattggaacacctgaggctcttgccctga
ggggaaaggctgctgtagcacaagcaaagatagcaaaccagctttacctc
aagaaattctctggcc

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