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GrainGenes Sequence Report: Ta.14222.1.S1_x_at

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Sequence
BF483989
Contig
Ta.14222.1.S1_at
Ta.14222.1.S1_x_at
NSFT03P2_Contig15451
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC320412
wEST map position
BF483989
NCBI UniGene
Ta.14222
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001054449.1 Os05g0112000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1BS
3AS
3BS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2306_H10_O20ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2306_H10_O20, mRNA sequence.
Other Name
WHE2306_H10_O20ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE2306_H10_O20
Probe
CFE39
WHE2306_H10_O20
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.14222.1.S1_at858WHE2AFFY
Ta.14222.1.S1_x_at858WHE2AFFY
TaAffx.98058.1.S1_at212WHE2AFFY
DNA
gcggaactgcttgcgtggggagctgatcggctccaaaggactctgctggg
agaattccctatgtggtggcacataagcgcaaccatggggcatgcgcagc
attgctgaacccttcctcggcagagcccatggtctggccttcccctctga
agttcatcagcgagctcgacccggaagccaaggcactgctggaagcggcc
ctgatggaggccaacagggagagggagaagaagatcttgaaggacgcaaa
gtgctcgccgcagtcccctttgcaatacgatgatgatcacatcgacgacg
acatgttctcggaggtgagcgacacggagctgtgctgcatctgcttcgac
caggcgtgcaccatcgaggtgcaggattgcgggcaccagatgtgcgcgcc
ctgcacgctggcgctgtgctgccacaacaagcccaacccggcgaccctga
ccctgccctcgccggcgtgcccgttctgccgcggcagcatctcgcggctg

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