Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Sequence Report: Ta.18.1.S1_at

[Submit comment/correction]

Sequence	BF483648
Contig	Ta.18.1.S1_at NSFT03P2_Contig18740
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC278017
wEST map position	BF483648
NCBI UniGene	Ta.18
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Translation initiation factor 4A'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6AS 6BS 7AL 7DL
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2336_C04_F08ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2336_C04_F08, mRNA sequence.
Other Name	WHE2336_C04_F08ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA019E1X
Clone	WHE2336_C04_F08
Probe	WHE2336_C04_F08
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.18.1.S1_at 954 WHE2AFFY [ Show all 9 ]
DNA	tttgtcctcgatgaagctgatgagatgctttcccgtggtttcaaggatca gatttatgacatcttccagcttctcccagggaagattcaagttggtgtct tctctgccaccatgccccctgaagcccttgagattacccgcaagttcatg aacaagccagtgaggatccttgtcaagagggatgagctcacccttgaggg tatcaagcagttctatgtcaatgtggagaaggaagagtggaagctcgaca cactgtgtgacctgtacgagactcttgccatcacccagagtgtcatcttt gtgaacacccgccgcaaggtggactggctcaccgacaagatgaggggcag ggaccacaccgtctccgccactcacggagacatggaccagaacactaggg acatcatcatgagggagttcagatcaggttcttcacgtgtgctcatcacc actgacctgcttgctcgtggtattgatgtccagcaagtgtcccttgtcat caactatgacctcc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.