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GrainGenes Sequence Report: BF483133

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Sequence
BF483133
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BF483133
NCBI UniGene
Ta.11865
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6AL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2315_B04_D07ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2315_B04_D07, mRNA sequence.
Other Name
WHE2315_B04_D07ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE2315_B04_D07
Probe
MAG1477
WHE2315_B04_D07
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.82096.1.S1_at50WHE2AFFY
DNA
gaggcgcgtctggaatctcccgctataagtaccgaggccacccacccccg
cccacgcgcgcgcggctccctcaaccagacgagacgagacgagacgcgag
gcccctcctcgattgatccgcgtcgacccccgctcgatcgtcatacgtca
ggcattaatcactcggagtttcatacgacaagatcaccgcggagatgtcg
ggctatgacgacaatgaaaccaacctagtggaggacgagtatgacgacct
tgatgatttcatcgtcggaagcgacgatgagggcgacaatgtggcccagg
aggacgaggaggagctgccggaggctgaggaggtagaagtggaagaagag
tatgaggaggaagaggaagaagagccgncggctggcacgcaggaaatcct
ttccttcagggagcaattaaaggccaaattaaggaaacaacatcagtcta
acggcgctgactacgggaaccctagctgttcgtcgtctgatcaaccgccg
gtcaggtccagatttggcaacttctttggcccgtccac

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