GrainGenes Sequence Report: BF482602

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Sequence

BF482602

Contig

NSFT03P2_Contig18871

Tracefile

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External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Gamma-gliadin'

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat pre-anthesis spike cDNA library

Tissue

Spike before anthesis

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2301-2304_I22_I22ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2301-2304_I22_I22, mRNA sequence.

Other Name

WHE2301-2304_I22_I22ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TA019E1X

Clone

WHE2301-2304_I22_I22

Probe

BF482602

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tccagcaaccacaacaaccttttccccagccccaacaacaattaccgcag
ccccaacaaccgcaacaatcattcccccaacaacaacggtcattcattca
accatctctacaacaacagttgaacccatgcaagaatatcctcttgcaac
aatgcaaacctgcgtcattggtgtcatccctctggtcaataatctggcca
caaagcgattgccaagtgatgcggcaacaatgctgccaacaactagcaca
gattcctcaacagctccagtgcgcagccatccatagcgtcgtgcattcca
tcatcatgcagcagcagcaacaacaacaacaacaacaaggcatgcatatc
tttctgccactatctcagcagcaacaggtgggtcaaggttctctagtcca
aggccagggcatcatccaaccacaacaaccagctcaattggaggcgatca
gatcattggtattgcaaactcttccatccatgtgcaacgtgtatgtccca
ccagagtgctccatcatgagggcaccatttgccagcatagtcgcgggcat
tggtggccaatgaaaaactgaagagctatactaataggtagatggatcat
cgttgctt


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