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GrainGenes Sequence Report: BF478737

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Sequence	BF478737
Contig	Ta.4599.1.S1_at NSFT03P2_Contig8103
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC281392
wEST map position	BF478737
NCBI UniGene	Ta.48399
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wr1.pk0067.h2:fis, full insert mRNA sequence'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1AS 1BS 1DS
Clone Library	Chinese Spring wheat drought stressed leaf cDNA library
Tissue	Leaf
Developmental Stage	Full tillering stage
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2013_A06_A11ZS Chinese Spring wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE2013_A06_A11, mRNA sequence.
Other Name	WHE2013_A06_A11ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA036E1X
Clone	WHE2013_A06_A11
Probe	WHE2013_A06_A11
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton , Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.4599.1.S1_at 321 WHE2AFFY Ta.4599.1.S1_at 111 WHE2AFFY Ta.10583.1.A1_at 113 WHE2AFFY Ta.10583.1.A1_at 70 WHE2AFFY
DNA	gtagctgcaatggatgttcttgttcccaagttggtgaattaatcggtgga agccaaagggaggagcgtcttgatattcttaagcaaaggatactggacgc ggatcttcctctggagccatatgagtggtacctggacctccgacgctttg gctcagtgaagcacagcgggttcggcct

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