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GrainGenes Sequence Report: BF478499

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Sequence
BF478499
Contig
Ta.872.1.S1_at
NSFT03P2_Contig11640
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC283614
wEST map position
BF478499
NCBI UniGene
Ta.872
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001045167.1 Os01g0913000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3BL
3DL
Clone Library
Chinese Spring wheat drought stressed leaf cDNA library
Tissue
Leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2009_D10_G19ZS Chinese Spring wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE2009_D10_G19, mRNA sequence.
Other Name
WHE2009_D10_G19ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA036E1X
Clone
WHE2009_D10_G19
Probe
WHE2009_D10_G19
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton , Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.872.1.S1_at882WHE2AFFY
[ Show all 12 ]
DNA
aaacaccgctctgcacaccaccaattcacaccacccgttgggggcgttac
cttccaaaactcctgcagccaatggcgttacgcctctccgtctcctcgcc
gcagagcacggcctcctcgccggccatctcctcatgccgccccgccgcct
gcggccgcttcctggcctgcgtggcggcttctcagaagaggagcctgatg
gcggtgtctgcctctgatgggagaggagtggctccggtgaaatccggcgg
cttggagacagccacgacgggagccggcgaggtggaagcgcccacagcgg
cggtggcggtgaccgggcaggtcacggaggtctgcaaggacaccttctgg
ccaattgtcaaggcagcaggggagaagcccgtcgtcctcgacatgtacac
ccaatggtgcggcccttgcaaagttattgcaccaaaattccaggagatgt
ctgagaaggaccacgacgtcgtgttcctcaagcttgattgcaatcaagac
aacaggcctcttgcaaaggagcttggcat

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