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GrainGenes Sequence Report: BF475103

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Sequence
BF475103
Contig
NSFT03P2_Contig18817
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC315647
NCBI UniGene
Ta.33558
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Alpha tubulin-3A'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat salt-stressed crown cDNA library
Tissue
Crown
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2112_G12_N24ZS Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2112_G12_N24, mRNA sequence.
Other Name
WHE2112_G12_N24ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA038E1X
Clone
WHE2112_G12_N24
Probe
BF475103
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
cgtcttcgtactcgcctctctctcgcgcttccgagctttgtccttcgtcc
tcctcccccgacctctccagcagcgtagcgtagcgcccgcttccgccgtt
ccgacccgccgccatgagggagtgcatctcgatccacatcggccaggctg
gtatccaggtcgggaacgcgtgctgggagctctactgcctcgagcatggc
attcagcctgatggacagatgcccggtgacaagactgttgggggaggtga
tgatgctttcaacaccttcttc

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