Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Sequence Report: NSFT03P2_Contig18368

[Submit comment/correction]

Sequence	BF475000
Contig	Ta.18.2.S1_x_at NSFT03P2_Contig18368
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC297370
wEST map position	BF475000
NCBI UniGene	Ta.54575
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_566469.1 EIF4A1 (eukaryotic translation initiation factor 4A-1); ATP-dependent helicase [Arabidopsis thaliana]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6AS 6BS 6DS 7DL
Clone Library	Wheat salt-stressed crown cDNA library
Tissue	Crown
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2104_E11_J22ZS Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2104_E11_J22, mRNA sequence.
Other Name	WHE2104_E11_J22ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA038E1X
Clone	WHE2104_E11_J22
Probe	BF475000 WHE2104_E11_J22
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.18.2.S1_x_at 1130 WHE2AFFY [ Show all 11 ]
DNA	agtatgacagcaagatgaccgagttactgaaccaaggtgacaccggggag tttttcacctcgtatgatgaggtgcacgaaagttttgatgacatgggcct ccaagagaaccttcttagaggcatctacgcttatggttttgagaagccat cagccattcagcaaagaggaattgttcccttctgcaagggtcttgatgtg attcagcaagctcagtctggaacaggaaaaactgccaccttctgttctgg aatcttgcagcagcttgactatggattggttgagtgccaggcattggtcc ttgctccgacccgtgagcttgcacagcagattgagaaggtcatgcgtgct cttggtgactacttaggtgtcaaggtgcatgcatgtgttggtggaacttc tgttcgtgaggaccaaaggattcttgccagtggtgtgcatgttgttgtcg gcacacctggtcgtgtgttcgatatgttgcgcaggcaatccctccgtcca gataacatcaagatgtttgtgttggatgaagctgatgaaatgctttcacg tggtttcaaggatcagatctatgatatcttccagcttcttccatcaaaga ttcaggttggggtgttctcagcaaccatg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.