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GrainGenes Sequence Report: BF474551

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Sequence	BF474551
Contig	NSFT03P2_Contig17830
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC282916
NCBI UniGene	Ta.54512
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001045534.1 Os01g0971500 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat salt-stressed crown cDNA library
Tissue	Crown
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2102_D02_G04ZS Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2102_D02_G04, mRNA sequence.
Other Name	WHE2102_D02_G04ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA038E1X
Clone	WHE2102_D02_G04
Probe	[Wcl41ct186cn284] {SpCl-296} BF474551
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	aggaggaggaggaggaggaggagaccatgtcgtcgtccggtggcaagctc ttcaccctggaggaggtcgccaagcacagctccaaggacgactgctggct cgtcatcggcggcaaggtgtacaatgtgaccaagtttcttgatgaccacc ctggaggcgacgacgtcttgctgtcttcaactgccaaggacgcgaccgac gacttcgaggacgtgggacacagcacaaccgcccgggcgatgatggacga gtactacgtcggcgagatcgacgcgaccacgatacccaccaaggtcaagt acacgccgcccaagcagccgcactacaaccaggacaagacccccgagttc gtcatcaagatcctccagttcttggtccccctcgcgatactgggcctggc cgtcgccgtacggatctacaccaaggcagactctgtctagcaccgtcatc ttataccagtcagtcagtcattcggaagtgtccaagagctgaataagttt tagtggggatttgcatattttgtaccagcgtcaccgtcctgtcatggaat ctgggaactgctctcgacccgtcgttgcgttatggttctactaagacgtc cgctgttgaatcgtgactcgggaaaaacggatagatacttggctata

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