GrainGenes Sequence Report: BF473511
Sequence
BF473511
Contig
NSFT03P2_Contig16575
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BF473511
NCBI UniGene
Ta.10655
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001049828.1 Os03g0296300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL 1DL
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0929_C02_E03ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0929_C02_E03, mRNA sequence.
Other Name
WHE0929_C02_E03ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0929_C02_E03
Probe
[Wcl722ct1553cn2117] CFE273 WHE0929_C02_E03
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.10655.1.S1_at 242 WHE2AFFY
[ Show all 8 ]
DNA
cttggccctcctccgcaaacttggggccgctgggacacaggctcgaggga
gacctggcctttcctcgccgcgcgagaagcttcaggcggcggcggcggcg
gctcaggtcttgctgataaggggacacgacagcgtactcccgagagtacg
tttgcgtcctccggtagcgggaagaggcaggcgccaccgggatccccttc
ccctgcctcccttaccgccgcctcttttcttggaggggaggaaaagaaag
gaaagccgagcaggacaagtagccagcgctcggcttcttctccaatccca
ttcatcgcctttccagtgatcctcacggcccgcagctagctccaccgtcg
tactaccgggagggggagatcagactgtgagatggcttcgccggagccat
ccgctggcggcgatgcggccagccagagtgctgcggtgcagccgctccag
ctgccaacgccggaggagatcaaggggcaggagatgatgaacaactgcgc
cgtccggagcgtcctcagtggagtcatgggtggtggtctcggtgtactta
tgggact
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BF473511
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