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GrainGenes Sequence Report: TaAffx.129390.1.S1_s_at

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Sequence	BF473204
Contig	Ta.4352.1.S1_a_at Ta.4352.2.S1_a_at TaAffx.129390.1.S1_s_at NSFT03P2_Contig9714
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC281380
wEST map position	BF473204
NCBI UniGene	Ta.48510
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'CRC-related protein'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4BL 4DL
Clone Library	Wheat 5-15 DAP spike cDNA library
Tissue	Spike
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0922_G09_M18ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0922_G09_M18, mRNA sequence.
Other Name	WHE0922_G09_M18ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA018E1X
Clone	WHE0922_G09_M18
Probe	MAG1436 WHE0922_G09_M18
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.4352.1.S1_a_at 1059 WHE2AFFY Ta.4352.1.S1_a_at 747 WHE2AFFY Ta.4352.2.S1_a_at 922 WHE2AFFY Ta.4352.2.S1_a_at 737 WHE2AFFY Ta.678.1.S1_at 52 WHE2AFFY
DNA	ggccgttccagggtgcactgactgcaggaggaaccagccgctgccaccgc tggcctcgccgacatcaagtgatgccagccccagagctccctttgttgtc aagcccccagagaagaaacaccgcctgccatctgcctacaatcgcttcat gagggaggaaatacaacgtatcaaagctgcaaagccagacatccctcaca gagaagccttcagcatggctgctaagaactgggcgaagtgcgaccctcgc tgctcatcgactgtctctgcttccaacagcgccccggagcccagaataat agtgcccggtcctcagctgcaggagagggctaccgagcaagtggttgaga gcttcgacatcttcaagcagatggagcgcagcgcctaaggaatcataagc atggtgggattaattagtactgctaccgcatgcatcggtcacttatcagc tagctcatcatcatcatccgtcgctatgcatatatataatgcataagcgc acgcgttttatttgtgtntggttacttcgttgctgctgctgctgtgtcgt taagttgatg

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