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GrainGenes Sequence Report: BF473119

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Sequence	BF473119
Contig	Ta.166.1.S1_a_at NSFT03P2_Contig7774
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC279574
wEST map position	BF473119
NCBI UniGene	Ta.166
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Cu/Zn superoxide dismutase'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	7AL 7BL 7DL
Clone Library	Wheat 5-15 DAP spike cDNA library
Tissue	Spike
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0927_H04_P07ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0927_H04_P07, mRNA sequence.
Other Name	WHE0927_H04_P07ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA018E1X
Clone	WHE0927_H04_P07
Probe	WHE0927_H04_P07
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.166.1.S1_a_at 722 WHE2AFFY Ta.166.1.S1_a_at 167 WHE2AFFY Ta.166.1.S1_a_at 101 WHE2AFFY TaAffx.52876.1.S1_at 48 WHE2AFFY
DNA	gaacgttcgtatcactggacttgctcctggacttcatggcttccactcct gagtttggtgacacgactaatggatgcatatcaacaggtccacattttaa cccaaacggcctgacacatggtgcaccagaagatgaagtccgtcatgcgg gtgacctgggaaacattgttgccaatgctgaaggtgtggcggagacaacc attgtcgatagccagattcctttgactggccctaatgcagttgttgggag agcgtttgttgttcatgagcttgaagatgacttgggaaaaggtgggcatg agctcagcctcagtactggaaatgctggtggaagacttgcatgtggtgtt gttggcctgaccccgttgtaggtcgctggtttccatctctggttttgcat ctccagtcatactggtttccatctctggttttgcatctccagtcatactt gtaacgacgccattctcgttttacctggatttcaaatatcggatgcttaa tagtttctgttgatcgtttgtatcagtgtaaagttgggtttatcaaataa atgtttgcacactttcgtctaataaaaaaaaaaaaaaaaa

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