GrainGenes Sequence Report: BF429039
Sequence
BF429039
Contig
NSFT03P2_Contig18875
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC316115
NCBI UniGene
Ta.28610
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Low-molecular-weight glutenin storage protein'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat heat stressed spike cDNA library
Tissue
Whole spike
Developmental Stage
Spikes at 5, 10, 15 and 20 days after anthesis
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1704_D05_H10ZS Wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1704_D05_H10, mRNA sequence.
Other Name
WHE1704_D05_H10ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA032E1X
Clone
WHE1704_D05_H10
Probe
BF429039
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttcctcgtctttgccctcctcgccgttgcggcgacaagtgcaattgcgca
gatggagactagatgcatccctggtttggagagaccatggcagcagcaac
cattaccaccacaacagacatttccacaacaaccactattttcacaacaa
caacaacaacaactatttcctcaacaaccatcattttcgcagcaacaacc
accattttggcagcaacaaccaccattttctcagcaacaaccaattctac
cacagcaaccaccattttcgcagcaacaacaactagttctaccgcaacaa
ccaccattttcacagcaacaacaaccagttttacctccacaacaatcacc
ttttccacaacaacaacaacaacaccaacagctggtgcaacaacaaatcc
ctgttgttcagccatccattttgcagcagctaaacccatgcaaggtattc
ctccagcagcagtgcagccctgtggcaatgccacaacgtcttgct

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BF429039
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