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GrainGenes Sequence Report: BF428914

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Sequence
BF428914
Contig
Ta.15268.1.S1_x_at
NSFT03P2_Contig18621
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC277239
NCBI UniGene
Ta.15268
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Pre-alpha-/beta-gliadin A-IV'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat heat stressed spike cDNA library
Tissue
Whole spike
Developmental Stage
Spikes at 5, 10, 15 and 20 days after anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1710_H08_O16ZS Wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1710_H08_O16, mRNA sequence.
Other Name
WHE1710_H08_O16ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA032E1X
Clone
WHE1710_H08_O16
Probe
BF428914
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
aagcagtgctcaatacaaatccaccatgaagacctttctgatccttgccc
tccttgctattgtagcaaccaccgccacaattgcagttagagttccagtg
ccacaattgcagccacaaaatccatctcagcaacaaccacaaaagcaagt
tccattggtacaacaacaacaatttccagggcagcaacaaccatttccag
ggcaacagccatatccgcagctgcaaccatttccatcacaacaaccatat
atgcagctgcaaccatttccgcagccgcaactaccatatccgcagccgca
actaccatatccgcagccgcaaccatttcgaccacaacaatcatatccac
aaccgcaaccacagtattcgcaaccacaacaaccaatttcgcagcagcag
cagcagc

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