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GrainGenes Sequence Report: BF428819

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Sequence
BF428819
Contig
Ta.24114.10.S1_x_at
NSFT03P2_Contig18609
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC311241
NCBI UniGene
Ta.63386
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_986604.1 AGL062Cp [Ashbya gossypii ATCC 10895]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat heat stressed spike cDNA library
Tissue
Whole spike
Developmental Stage
Spikes at 5, 10, 15 and 20 days after anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1711_G01_N01ZS Wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1711_G01_N01, mRNA sequence.
Other Name
WHE1711_G01_N01ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA032E1X
Clone
WHE1711_G01_N01
Probe
BF428819
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
catcatcaacaacaacaacaacaactacaacaacaacaacaacaacaaca
acaaccgtcgagccatgtctcctaccaccatcctcaccaacaatatccat
catgccatggcttattccagccatctcagcaaaacccacaagcccatggc
tttgtgcaacctcagcaactgccgcagttcaaggaaataacgaacctaac
gctgcacacgctaccatcaatgtgcaatgtctacatccctccatattggt
cgaccaccattgcgccatttggcatcatgagtactaactgagaagagaag
aactctattactagatatatgaaacaccgttttcttaaaacatggtttgg
tcgttgtagcggtgaaaaataaagtgacatgcactatcatgtaagaaccc
gaactagactatttcaaacttggaaataaaagacaaacacagggtcttgt
ctgcaaaaaaaaaaa

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