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GrainGenes Sequence Report: NSFT03P2_Contig8439

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Sequence
BF428729
Contig
NSFT03P2_Contig8439
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC289638
wEST map position
BF428729
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
TAM W-101
Species
Triticum aestivum
Cultivar
TAM W101
TAM W-101
Chromosome
6AL
6DL
Clone Library
Wheat drought stressed leaf cDNA library
Tissue
Leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1412_F02_L04ZS Wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE1412_F02_L04, mRNA sequence.
Other Name
WHE1412_F02_L04ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA027E1X
Clone
WHE1412_F02_L04
Probe
MAG1474
WHE1412_F02_L04
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.19822.2.S1_at96WHE2AFFY
Ta.15768.1.A1_at46WHE2AFFY
DNA
gccccctgcacctgcctcaccacaaggaggaggacaaggagggggaggag
aagcagcacggccatgaccacggtcacggtcacggtcacgaccacaacca
tggccacggccacgaccacaaccacaaccacagggagcacgccggcggga
gggggctggacatcgacatggacctaccggcgtacgtcaacatgggctcc
atcaccaccgcgggcgttctcgactggaggtacc

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