GrainGenes Sequence Report: BF293881

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Sequence

BF293881

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum monococcum

Keyword

EST

Species

Triticum monococcum

Cultivar

DV92

Clone Library

Triticum monococcum vegetative apex cDNA library

Tissue

Vegetative shoot apex

Developmental Stage

Three weeks-old plants

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2253_B03_D05ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE2253_B03_D05, mRNA sequence.

Other Name

WHE2253_B03_D05ZE [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli XLOLR

Clone

WHE2253_B03_D05

Probe

[Wcl127ct584cn929]

{SpCl-192}

BF293881

Remark

DB_xref: taxon:4568

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene T3 primer.

Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tcggcacgagggccatggagtcgcagacgatccgccacatgatcgaggac
gactgcgccgacaacggcatcccgctccccaacgtcaactccaagatcct
ctccaaggtcatcgagtactgcaacaagcacgtccaggccaagcccgccg
acggggccgcggccggagccggagcctcggacgcctccgccgccgccccc
gccgcccccgccgaggacctcaagaactgggacgccgagttcgtcaaggt
cgaccaggccaccctcttcgacctcatcctggctgccaactacctcaaca
tcaaggggctgctggacctgacctgccagactgttgccgacatgatcaag
ggcaagaccccagaggagatccgcaagaccttcaacatcaagaatgactt
tacccccgaggaggaggaggagatcc


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