GrainGenes Sequence Report: TaAffx.95561.1.S1

GrainGenes Sequence Report: TaAffx.95561.1.S1_at

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Sequence

BF293856

Contig

TaAffx.95561.1.S1_at

NSFT03P2_Contig10573

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum turgidum

Keyword

EST

Germplasm

Langdon-16

Species

Triticum turgidum

Triticum turgidum ssp. durum

Cultivar

Langdon-16

Clone Library

Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library

Tissue

All tissues

Developmental Stage

Different growth stages

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2153_G07_N13ZS Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library Triticum turgidum cDNA clone WHE2153_G07_N13, mRNA sequence.

Other Name

WHE2153_G07_N13ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TT039E1X

Clone

WHE2153_G07_N13

Probe

[Wcl896ct1766cn2358]

{SpCl-268}

Remark

DB_xref: taxon:4571

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at North Dakota State University (Kianian, Otto, Simons). Tissues collected from seven-day etiolated seedling leaf, stem, root and seed; leaf from plant at fourth leaf stage; spike from pre-anthesis through 20 days after anthesis; flag leaf; leaf and stem tissue from tillers. and root. Total RNA and poly(A) RNA were prepared from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ttgccttctttggcgacgcgccccccccttccctttttctgtagcacctt
tgagctttatgctctcctcccattactttagcaagaaatctcaggacaaa
tcctgcgggactttagttagagagagaccgatggcggcggcgagcgtcgg
cggcggagcggcgccgttcgtgtggaagacgtaccggatggtggaggacc
ccgggacggacggggtcatcgggtgggggaaggggaacaatagcttcgtc
gtcgccgaccccttcgtcttctcgcagaccatgctccccgcg


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