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GrainGenes Sequence Report: BF293263

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Sequence
BF293263
Contig
Ta.22773.1.S1_x_at
NSFT03P2_Contig11654
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BF293263
DB Remark
Locus Source: Triticum turgidum
Keyword
EST
Germplasm
Langdon-16
Species
Triticum turgidum
Triticum turgidum ssp. durum
Cultivar
Langdon-16
Chromosome
6BL
6DL
Clone Library
Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library
Tissue
All tissues
Developmental Stage
Different growth stages
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2151_E01_I01ZS Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library Triticum turgidum cDNA clone WHE2151_E01_I01, mRNA sequence.
Other Name
WHE2151_E01_I01ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TT039E1X
Clone
WHE2151_E01_I01
Probe
BF293263
WHE2151_E01_I01
Remark
DB_xref: taxon:4571
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at North Dakota State University (Kianian, Otto, Simons). Tissues collected from seven-day etiolated seedling leaf, stem, root and seed; leaf from plant at fourth leaf stage; spike from pre-anthesis through 20 days after anthesis; flag leaf; leaf and stem tissue from tillers. and root. Total RNA and poly(A) RNA were prepared from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.22773.1.S1_x_at926WHE2AFFY
[ Show all 14 ]
DNA
tggcgaacaaacacttgtccctctcgctcttcctcgtcctccttggcctg
tcggccagcttggcctccgggcaagtcctgtttcagggtttcaactggga
gtcgtggaagcacaatggcgggtggtacaacttcctgatgggcaaggtgg
acgacatcgccgccgccggcatcacgcacgtctggctccctccggcgtcg
cagtccgtcgctgagcaaggctacatgccgggccggctctacgacctgga
cgcctccaagtacggcaacaaggcgcagctcaagtccctcatcggggcgc
tccacggcaagggcgtcaaggccatcgccgacatcgtcatcaaccaccgc
acggcggagcacaaggacggccggggcatctactgcatcttcgaaggcgg
cacccctgacgcccgcctcgactggggcccccacatgatctgccgtgacg
accggccctacgccgacggcaccggcaacccggacaccggcgccgacttc
ggggccgcccccgacatcgaccacctcaacccgcgcgtccagaaggagct
cgtcgagtggctcaactggctcaagac

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