GrainGenes Sequence Report: BF292470

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Sequence

BF292470

Contig

NSFT03P2_Contig10260

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Aegilops speltoides

Keyword

EST

Species

Aegilops speltoides

Cultivar

F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1 )

F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)

Clone Library

Aegilops speltoides anther cDNA library

Tissue

Anther

Developmental Stage

Premeiotic anthers

Data Source

genbank	Release 135, Apr 15 2003
genbank	Downloaded 2008-2009

Title

WHE2214_B10_C20ZS Aegilops speltoides anther cDNA library Aegilops speltoides cDNA clone WHE2214_B10_C20, mRNA sequence.

Other Name

WHE2214_B10_C20ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

AS040E1X

Clone

WHE2214_B10_C20

Remark

DB_xref: taxon:4573

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons , Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

agcttttcgtctcgtctcgtcttctccccttttcgagatctcgccagttt
ggattttgaagctcagcttcttgacacgcatctgatctggtagctatttg
tatgtgttcacaagacattcgacatgaagcttttagaatacaccccgttc
gaccgtgtaaatgtgttcctcgatcaactaaaccttggtgattgtacaat
taggggaagccttgaagccttctcatgtaagcatgcaggaaatgatcgcc
ggctctcaatcagcctggaacatgagattcttgattaccttggcaagtct
tctgatagtgatcctccttcacctgtggagcatttgtcttgtagatctag
ccggaaaacgttgatatatctagttctcactcttggccatatgtatccag
attatgatttcagtgctgttcgggcacacctattcttcaaagaagaagac
atggaaagtttcaagcagatggtagacaactacttatcg


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