GrainGenes Sequence Report: BF258214

HVSMEf0015B24f Hordeum vulgare seedling root EST library HVcDNA0007 (Etiolated and unstressed) Hordeum vulgare subsp. vulgare cDNA clone HVSMEf0015B24f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HVSMEf0015B24f

Probe

[BF258214.1]

{SpCl-541}

BF258214

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 461; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 652.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling roots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling roots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

gctgttgacaaggtctgacaaagctgggcgcctgataggaatgctgatcc
caggggatatgggggtattcagaccacaggtctcgactcagcagccacag
atgcagccacagcagcagcagcagctgcaacagcagcagcaccaaataca
gcagcaacagcagcaactacagcaactgcaacagcagcaactacagcagc
accaaatgcaaatgcagcctcaaggccagcaacttcagcagcagcagcag
atgcagcaaatgcaacagcaacaacaacagatgcagcaaatgcgacacca
acaacagcggcagcagattcaacagcagcagcagatgcagcagcagcagc
aacaacaacagcaacagatgcagcagatgcagcagcagcagcagcaacag
cctgaacaactccagcagcagccccagatggtgggtacagggatgggtca
gcaacaattcatgcaggggcatggtcgggcggtgcagatgatgcaaggaa
agatcgcgccacagggcccaggcaacatgtctgggggaggctacctatct
tgaaaatatagcgtgctagcaggacactcattgggttcatgatgcccctt
ggaaatttgggggtaaagtctcccacccaactagggtgggggttttgggc
ttatgtacagagcattgcgagtctggctgctgagggggagtttgcttatt
atcccatgtctaacctggagttgaagaattcacccaaatttttctatttg
gggaaatgggggggctcc


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