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GrainGenes Sequence Report: Ta.11599.2.S1_a_at

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Sequence
BF201533
Contig
Ta.11599.2.S1_a_at
NSFT03P2_Contig15512
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC295521
wEST map position
BF201533
NCBI UniGene
Ta.11599
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001060381.1 Os07g0634400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2BS
2DS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1771_F11_K21ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1771_F11_K21, mRNA sequence.
Other Name
WHE1771_F11_K21ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE1771_F11_K21
Probe
WHE1771_F11_K21
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.11599.3.S1_at367WHE2AFFY
Ta.11599.3.S1_at48WHE2AFFY
TaAffx.12415.1.A1_at256WHE2AFFY
Ta.11599.2.S1_a_at113WHE2AFFY
Ta.14491.1.S1_at62WHE2AFFY
DNA
tcacctcacctgcgtagcgtgcgtgcagctggtgccctgtcttccactcc
tccgttcatcgtctagacggtgtccactccgtgaagatggaatcctacgc
caacggagccaaattcgattgcctgctgtttgacatggatgacaccctct
acccactgagcttagggatcaacctagcctgccgcaagaacatccaagac
tacatgctgaacaagcttcagatcgaggagagcctggtccccaagatgtg
cctggatctgtacaaagaatacggaaccacgatggccggtctgaagctta
tgggctacgatttcgactacgatgagttccacgccagcgtgcacggtaaa
ctgccctacgagaagctgaagccggaccccgtcctgaggaacctgctgct
ttcgatgccacagaggaaaatcatattcaccaactccgacgaggctcacg
cggcgaccgtcctggagaagatgggcctggaggggtgctttgagggaatc
atatgcttcgagaccctgaacc

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