GrainGenes Sequence Report: BF201151
Sequence
BF201151
Contig
Ta.1376.1.S1_at NSFT03P2_Contig15941
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC282831
wEST map position
BF201151
NCBI UniGene
Ta.48536
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wr1.pk0122.e5:fis, full insert mRNA sequence'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL 3BL 3DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1769_C07_F13ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1769_C07_F13, mRNA sequence.
Other Name
WHE1769_C07_F13ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE1769_C07_F13
Probe
[Wcl776ct1627cn2203] {SpCl-209} WHE1769_C07_F13
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.1376.1.S1_at 472 WHE2AFFY TaAffx.85773.1.S1_x_at 62 WHE2AFFY TaAffx.85773.1.S1_at 62 WHE2AFFY TaAffx.8239.1.S1_at 46 WHE2AFFY
DNA
tccggccgggcgggatgttcgtgcagcgcagggacggcgaggagggcccc
accgtcaggctcagggtgtcccacggccccgccctgcgcgacgtcttcgt
gccggcgcaggccaccttcggtgaactgaagaggatcctcacccagacta
ctggcctggagcctgagagacaaaggctctttttccgtggtaaggagaag
agggacgatgaattcctgcacgcatcaggcatccaaggatggagcaaaat
tgcttctgcttgaaaaacatgtccctgccaatgtggaacaaaaggttaag
ccaatgatgatggat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BF201151
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