Query (optional)   in Class  

GrainGenes Sequence Report: NSFT03P2_Contig18561

[Submit comment/correction]

Sequence
BF200716
Contig
NSFT03P2_Contig18561
Tracefile
[ Download ]
[ View ]
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC279834
wEST map position
BF200716
NCBI UniGene
Ta.54071
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001049162.1 Os03g0180400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4DL
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0821-0824_I19_I19ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0821-0824_I19_I19, mRNA sequence.
Other Name
WHE0821-0824_I19_I19ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA016E1X
Clone
WHE0821-0824_I19_I19
Probe
WHE0821-0824_I19_I19
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.6475.1.S1_at527WHE2AFFY
TaAffx.57911.2.S1_at285WHE2AFFY
TaAffx.57911.2.S1_at72WHE2AFFY
TaAffx.57911.1.S1_at147WHE2AFFY
DNA
caggcgacgcaccacccaggtccagctccggcgagatctccggcgaccct
tcgagatgagccgcggatcgggcgcgggctacgaccgccacatcaccatc
ttctccccgaggtcgcctctaccaagtcgagtacgcgttcaaggcggtca
agtcggcgggggtcacctcgatcggcgtccgcggcaaggactccgtctgc
gtcgtcacccaaaagaaagtcccggacaagttgctggatgacaccagcat
cacgcacctcttctcgattaccaagtacaccggcttgcttgccactggcc
tcacagctgatgc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.