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GrainGenes Sequence Report: TaAffx.25629.1.S1_s_at

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Sequence
BF200672
Contig
Ta.593.3.S1_a_at
TaAffx.25629.1.S1_s_at
NSFT03P2_Contig2767
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC279870
NCBI UniGene
Ta.593
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Vacuolar H+-pyrophosphatase'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0821-0824_A21_A21ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0821-0824_A21_A21, mRNA sequence.
Other Name
WHE0821-0824_A21_A21ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA016E1X
Clone
WHE0821-0824_A21_A21
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.1409.1.S1_x_at113WHE2AFFY
DNA
cagccctgccattacagcgtgggcaagacatgcaagcctgctcttgctaa
cgccgcgttcagcacaattgcctttgtgctgggtgcggtcacctctctgg
tgtctggtttccttggaatgaagatcgcgacttacgcgaatgccaggaca
actcttgaggcaaggaagggtgttgggaaggcgtttaccgttgctttccg
ctccggtgctgtgatgggcttccttcttgctgcaagtggcctttttgttc
tttacgttgcaatcaacttgtttgggctttattatggtgatgactgg

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